Caffeine synthase gene from tea leaves

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Extraction and isolation of Caffeine from tea leaves

General background and overview of the experiment: Humankind has historically used advantageous compounds derived from plants and animals. For instance, various extracts from plants have been used as teas, potions, medicines and poisons. Though these extracts can contain a mixture of many different chemicals, often only one or few are responsible for the activity of the extract. For decades suc...

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[Frontiers in Bioscience 9, 1833-1842, May 1, 2004] 1833 CAFFEINE SYNTHASE AND RELATED METHYLTRANSFERASES IN PLANTS

1. Abstract 2. Introduction 3. N-methyltransferases involved in caffeine biosynthesis 3.1. N-methyltransferase activity in cell free preparations 3.2. Purification of caffeine synthase from tea leaves 3.3. Molecular cloning of caffeine synthase gene from tea leaves 3.4. The orthologous genes in coffee 3.5. The characteristic future of N-methyltransferase in theobromineaccumulating species 4. Ca...

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Transfer of 226Ra and 137Cs from tea leaves to drinking tea

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Separation of polyphenols and caffeine from the acetone extract of fermented tea leaves (Camellia sinensis) using high-performance countercurrent chromatography.

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In this work, we studied the biosynthesis of caffeine by examining the expression of genes involved in this biosynthetic pathway in coffee fruits containing normal or low levels of this substance. The amplification of gene-specific transcripts during fruit development revealed that low-caffeine fruits had a lower expression of the theobromine synthase and caffeine synthase genes and also contai...

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ژورنال

عنوان ژورنال: Nature

سال: 2000

ISSN: 0028-0836,1476-4687

DOI: 10.1038/35023072